Improve the light conditions Protect the melon when it is covered with a drop-free film and keep the film clean. Try to get the maximum light transmittance and make the soil accumulate more heat. Release the reduced plants, properly dilute the plants, pay attention to pruning, tying the vines, picking the heart, and comprehensively improve the lighting conditions in the field. Controlling the temperature of the melon in the melon period, the temperature difference between day and night is large, which is conducive to the accumulation of nutrients during the day and the reduction of nighttime consumption. The daytime temperature should be controlled at 26 °C ~ 28 °C, and the nighttime temperature should be controlled at 10 °C ~ 12 °C. In case of abnormal weather, it is necessary to take remedial measures in time. Scientific application of fertilizer and fertilizer should be carried out with more organic fertilizers. In particular, it is necessary to apply high-quality organic fertilizers such as chicken manure and cake fertilizer which are fully decomposed and harmlessly treated. Do not apply excessive nitrogen fertilizer. Reasonable watering, keep the soil with enough water, watering a small number of times, it is not easy to water on rainy days, it is better to water on sunny days. Keep the proper melon position and do not leave the root melon. Generally, the bitterness is mostly on the root melon. Because the root melon is close to the root of the plant, the effective function leaves are few, the nutrient condition is poor, and if the fruit is in a low temperature period, the lack of light and malnutrition In the case of the case, it is easy to make the melon skin thick and bitter, but the melon position cannot be too far from the root. Do not damage the roots at the right time, do not hurt the roots or minimize the root damage during colonization and field management, to avoid bitterness. The correct application of sitting melon is suitable for harvesting, using 2 to 3 kg (diluted 400 to 600 times) per 5 g of water, the application concentration is related to the ambient temperature, and the high temperature should be appropriately lowered; the temperature is lower than 15 °C should not be applied. After applying the melon, you must wait until the melon is fully mature before harvesting, so that the bitterness is fully transformed. It is recommended to reduce the administration of sitting guar or plant growth regulators. If grafting is cultivated, it is necessary to pay attention to the choice of rootstock varieties. Strengthen pest and disease prevention and control. Pests and diseases in summer maize ear should focus on brown spot disease and corn borer. Brown spot disease can be controlled by 12.5% ​​straw or 12.5% ​​rusting foliar spray; corn mash can be filled with phoxim granules at the big bell stage or with 4.5% beta-cypermethrin emulsion or 50% phoxim emulsifiable concentrate Heart leaf spray is used for prevention and treatment. Disclaimer: Some articles on this website are transferred from the Internet. If legal rights of third parties are involved, please inform this website. phone Magnetic Bead Nucleic Acid Extraction Reagent
The magnetic bead method nucleic acid extraction kit is a high-tech product that combines biological science and nanomaterial science. It is a major breakthrough in my country's nucleic acid extraction and purification technology. .
Magnetic bead method for nucleic acid extraction has incomparable advantages over traditional DNA extraction methods, which are mainly reflected in: 1. It can realize automatic and large-scale operation. At present, there are 96-well nucleic acid automatic extraction instruments, and the extraction time of one sample can be realized. The processing of 96 samples complies with the high-throughput operation requirements of biology, enabling rapid and timely response to infectious disease outbreaks, which makes traditional methods unmatched; â‘¡ The operation is simple and time-consuming, and the entire extraction process has only four steps , most of them can be completed within 36-40 minutes; â‘¢ Safe and non-toxic, no toxic reagents such as benzene and chloroform in traditional methods are used, and the damage to experimental operators is minimized, which fully conforms to modern environmental protection concepts; â‘£ Magnetic beads and nucleic acids The specific binding of the extracted nucleic acid results in high purity and high concentration of the extracted nucleic acid.
According to the same principle as the silica membrane spin column, the superparamagnetic silica nano-magnetic beads are prepared after the surface of the superparamagnetic nanoparticles is modified and modified by nanotechnology. The magnetic beads can specifically recognize and efficiently bind to nucleic acid molecules on a microscopic interface. Using the superparamagnetic properties of silica-coated nanomagnetic microspheres, under the action of Chaotropic salts (guanidine hydrochloride, guanidine isothiocyanate, etc.) and an external magnetic field, samples from blood, animal tissues, food, pathogenic microorganisms, etc. The isolated DNA and RNA can be used in clinical disease diagnosis, blood transfusion safety, forensic identification, environmental microbial testing, food safety testing, molecular biology research and other fields.
Magnetic bead nucleic acid extraction can generally be divided into four steps: lysis-binding-washing-elution.
Genetic testing will surely become a new symbol of the development of the biological industry. The emergence of high-throughput, automated nucleic acid extraction methods will reduce the labor cost of genetic testing, make large-scale testing a reality, and make it possible for genetic testing to reach ordinary people.
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